Coding

Part:BBa_K3113100:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-09-26)


Gag HIV-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 607


Design Notes

Silent mutations to remove illegal restriction sites. A312T, A318G, A630T, T720A


Source

This sequence was provided to the team by PhD student Christoph Gruber of the Helmholtz Center Munich.

References